1
缁
1
锛庣敓鐗╁寲瀛︾爺绌剁殑瀵硅薄鍜屽唴瀹规槸浠�涔堬紵
瑙ｇ瓟锛氱敓鐗╁寲瀛︿富瑕佺爺绌?/p>
:
锛?/p>
1
锛夌敓鐗╂満浣撶殑鍖栧缁勬垚銆佺敓鐗╁垎瀛愮殑缁撴瀯銆佹�ц川鍙婂姛鑳斤紱
锛?/p>
2
锛夌敓鐗╁垎瀛愬垎瑙ｄ笌鍚堟垚鍙婂弽搴旇繃绋嬩腑鐨勮兘閲忓彉鍖栵紱
锛?/p>
3
锛夌敓鐗╅仐浼犱俊鎭殑鍌ㄥ瓨銆佷紶閫掑拰琛ㄨ揪锛?/p>
锛?/p>
4
锛夌敓鐗╀綋鏂伴檲浠ｈ阿鐨勮皟鑺備笌鎺у埗銆?/p>
2
锛庝綘宸茬粡瀛﹁繃鐨勮绋嬩腑鍝簺鍐呭涓庣敓鐗╁寲瀛︽湁鍏炽�?/p>
鎻愮ず锛氱敓鐗╁寲瀛︽槸鐢熷懡绉戝鐨勫熀纭�瀛︾锛屾敞鎰忎粠涓嶅悓鐨勮搴︼紝鍘荤悊瑙ｅ苟杩愮敤鐢熺墿鍖栧鐨?/p>
鐭ヨ瘑銆?/p>
3
锛庤鏄庣敓鐗╁垎瀛愮殑鍏冪礌缁勬垚鍜屽垎瀛愮粍鎴愭湁鍝簺鐩镐技鐨勮渚撱�?/p>
瑙ｇ瓟锛氱敓鐗╁ぇ鍒嗗瓙鍦ㄥ厓绱犵粍鎴愪笂鏈夌浉浼肩殑瑙勪緭鎬с�傜⒊銆佹阿銆佹哀銆佹爱銆佺７銆佺～绛?/p>
6
绉嶆槸铔?/p>
鐧借川銆佹牳閰搞�佺硸鍜岃剛鐨勪富瑕佺粍鎴愬厓绱犮�傜⒊鍘熷瓙鍏锋湁鐗规畩鐨勬垚閿�ц川锛屽嵆纰冲師瀛愭渶澶栧眰鐨?/p>
4
涓?/p>
鐢靛瓙鍙娇纰充笌鑷韩褰㈡垚鍏变环鍗曢敭銆佸叡浠峰弻閿拰鍏变环涓夐敭锛岀⒊杩樺彲涓庢爱銆佹哀鍜屾阿鍘熷瓙褰㈡垚鍏变环
閿�?/p>
纰充笌琚敭鍚堝師瀛愬舰鎴?/p>
4
涓叡浠烽敭鐨勬�ц川
,
浣垮緱纰抽鏋跺彲褰㈡垚绾挎�с�?/p>
鍒嗘敮浠ュ強鐜姸鐨勫绉嶅
鎬х殑鍖栧悎鐗┿�傜壒娈婄殑鎴愰敭鎬ц川閫傚簲浜嗙敓鐗╁ぇ鍒嗗瓙澶氭牱鎬х殑闇�瑕併�傛爱銆佹哀銆佺～銆佺７鍏冪礌鏋勬垚浜?/p>
鐢熺墿鍒嗗瓙纰抽鏋朵笂鐨勬皑鍩猴紙
-NH2
锛?/p>
銆佺緹鍩猴紙
-OH
锛?/p>
銆佺景鍩猴紙锛?/p>
銆佺晶鍩猴紙
-COOH
锛?/p>
銆佸矾鍩猴紙
-SH
锛?/p>
銆佺７
閰稿熀
锛?/p>
-PO4
锛?/p>
绛夊姛鑳藉熀鍥€�?/p>
杩欎簺鍔熻兘鍩哄洟鍥犳爱銆?/p>
纭拰纾锋湁鐫�鍙彉鐨勬哀鍖栨暟鍙婃爱鍜屾哀鏈夌潃杈冨己
鐨勭數璐熸�ц�屼笌鐢熷懡鐗╄川鐨勮澶氬叧閿綔鐢ㄥ瘑鍒囩浉鍏炽�?/p>
鐢熺墿澶у垎瀛愬湪缁撴瀯涓婁篃鏈夌潃鍏卞悓鐨勮寰嬫�с�傜敓鐗╁ぇ鍒嗗瓙鍧囩敱鐩稿悓绫诲瀷鐨勬瀯浠堕�氳繃涓�瀹氱殑
鍏变环閿仛鍚堟垚閾剧姸锛?/p>
鍏朵富閾鹃鏋跺憟鐜板懆鏈熸�ч噸澶嶃�?/p>
鏋勬垚铔嬬櫧璐ㄧ殑鏋勪欢鏄?/p>
20
绉嶅熀鏈皑鍩洪吀銆?/p>
姘?/p>
鍩洪吀涔嬮棿閫氳繃鑲介敭鐩歌繛銆傝偨閾惧叿鏈夋柟鍚戞�э紙
N
绔啋
C
绔級
,
铔嬬櫧璐ㄤ富閾鹃鏋跺憟
"
鑲藉崟浣?/p>
"
閲嶅锛?/p>
鏍搁吀鐨勬瀯浠舵槸鏍歌嫹閰?/p>
,
鏍歌嫹閰搁�氳繃
3
鈥?/p>
,
5
鈥?/p>
-
纾烽吀浜岄叝閿浉杩烇紝鏍搁吀閾句篃鍏锋湁鏂瑰悜鎬?/p>
(5
鈥层�佲啋
3
鈥?/p>
),
鏍搁吀鐨勪富閾鹃鏋跺憟
"
纾烽吀
-
鏍哥硸
锛堟垨鑴辨哀鏍哥硸锛?/p>
"
閲嶅锛?/p>
鏋勬垚鑴傝川鐨勬瀯浠舵槸鐢樻补銆?/p>
鑴傝偑閰?/p>
鍜岃儐纰憋紝鍏堕潪鏋佹�х儍闀块摼涔熸槸涓�绉嶉噸澶嶇粨鏋勶紱鏋勬垚澶氱硸鐨勬瀯浠舵槸鍗曠硸锛屽崟绯栭棿閫氳繃绯栬嫹閿浉
杩烇紝娣�绮夈�佺氦缁寸礌銆佺硸鍘熺殑绯栭摼楠ㄦ灦鍧囧憟钁¤悇绯栧熀鐨勯噸澶嶃�?/p>
2
铔嬬櫧璐ㄥ寲瀛?/p>
1
锛庣敤浜庢祴瀹氳泲鐧借川澶氳偨閾?/p>
N
绔�?/p>
C
绔殑甯哥敤鏂规硶鏈夊摢浜涳紵鍩烘湰鍘熺悊鏄粈涔堬紵
瑙ｇ瓟锛?/p>
锛?/p>
1
锛?/p>
N-
鏈娴嬪畾娉曪細甯搁噰鐢ㄢ�曚簩纭濆熀姘熻嫰娉曘�?/p>
Edman
闄嶈В娉曘�佷腹纾洪叞姘硶銆?/p>
鈶犫�曚簩纭濆熀姘熻嫰
(DNFB
鎴?/p>
FDNB)
娉曪細澶氳偨鎴栬泲鐧借川鐨勬父绂绘湯绔皑鍩轰笌鈥曚簩纭濆熀姘熻嫰锛堚�?/p>
DNFB
锛夊弽搴旓紙
Sanger
鍙嶅簲锛?/p>
锛岀敓鎴?/p>
DNP
鈥曞鑲芥垨
DNP
鈥曡泲鐧借川銆傜敱浜?/p>
DNFB
涓庢皑鍩哄舰鎴愮殑閿
閰告按瑙ｈ繙姣旇偨閿ǔ瀹氾紝
鍥犳
DNP
鈥曞鑲界粡閰告按瑙ｅ悗锛?/p>
鍙湁
N
鈥曟湯绔皑鍩洪吀涓洪粍鑹?/p>
DNP
鈥曟皑鍩洪吀
琛嶇敓鐗╋紝鍏朵綑鐨勯兘鏄父绂绘皑鍩洪吀銆?/p>
鈶?/p>
涓圭：閰版隘
(DNS)
娉曪細
澶氳偨鎴栬泲鐧借川鐨勬父绂绘湯绔皑鍩轰笌涓庝腹纾洪叞姘?/p>
锛?/p>
DNS
鈥?/p>
Cl
锛?/p>
鍙嶅簲鐢熸垚
DNS
鈥曞鑲芥垨
DNS
鈥曡泲鐧借川銆傜敱浜?/p>
DNS
涓庢皑鍩哄舰鎴愮殑閿閰告按瑙ｈ繙姣旇偨閿ǔ瀹氾紝鍥犳
DNS
鈥曞
鑲界粡閰告按瑙ｅ悗锛?/p>
鍙湁
N
鈥曟湯绔皑鍩洪吀涓哄己鐑堢殑鑽у厜鐗╄川
DNS
鈥曟皑鍩洪吀锛?/p>
鍏朵綑鐨勯兘鏄父绂绘皑鍩洪吀銆?/p>
鈶?/p>
鑻紓纭鞍閰歌剛锛?/p>
PITC
鎴?/p>
Edman
闄嶈В锛夋硶锛氬鑲芥垨铔嬬櫧璐ㄧ殑娓哥鏈姘ㄥ熀涓庡紓纭鞍閰?/p>
鑻叝锛?/p>
PITC
锛夊弽搴旓紙
Edman
鍙嶅簲锛?/p>
锛岀敓鎴愯嫰姘ㄥ熀纭敳閰板鑲芥垨铔嬬櫧璐ㄣ�傚湪閰告�ф湁鏈烘憾鍓備腑鍔犵儹
鏃讹紝
N
鈥曟湯绔殑
PTC
鈥曟皑鍩洪吀鍙戠敓鐜寲锛岀敓鎴愯嫰涔欏唴閰扮～鑴茬殑琛嶇敓鐗╁苟浠庤偨閾句笂鎺変笅鏉ワ紝闄ゅ幓
N
鈥曟湯绔皑鍩洪吀鍚庡墿涓嬬殑鑲介摼浠嶇劧鏄畬鏁寸殑銆?/p>
鈶?/p>
姘ㄨ偨閰舵硶锛氭皑鑲介叾鏄竴绫昏偨閾惧鍒囬叾鎴栧彨澶栬偨閰讹紝鑳戒粠澶氳偨閾剧殑
N
绔�愪釜鍦板悜閲屽垏銆?/p>
鏍规嵁涓嶅悓鐨勫弽搴旀椂闂存祴鍑洪叾姘磋В閲婃斁鐨勬皑鍩洪吀绉嶇被鍜屾暟閲忥紝鎸夊弽搴旀椂闂村拰娈嬪熀閲婃斁閲忎綔鍔ㄥ姏
瀛︽洸绾匡紝灏辫兘鐭ラ亾璇ヨ泲鐧借川鐨?/p>
N
绔畫鍩哄簭鍒椼�?/p>
锛?/p>
2
锛?/p>
C
鈥曟湯绔祴瀹氭硶锛氬父閲囩敤鑲艰В娉曘�佽繕鍘熸硶銆佺晶鑲介叾娉曘�?/p>