涓�銆佽繃姘у寲鐗╅叾锛?/p>
POD
锛夋椿鎬х殑娴嬪畾
POD
娴嬪畾鍙傜収鏉庡悎鐢熺瓑锛?/p>
2003
锛夌殑鎰堝垱鏈ㄩ厷娉曟柟娉曡繘琛屾祴瀹氾紝鐣ュ姞鏀瑰姩銆?/p>
娴嬪畾锛氱О鍙栨牱鍝?/p>
0.5g
锛屽姞鍏?/p>
5mL
1
锛?/p>
15
mol
锛?/p>
L PH=7.0
鐨勭７閰哥紦鍐叉憾娑诧紝鍐版荡
鐮旂（鎴愬寑娴嗭紝
4
鈩冩潯浠朵笅
12000r/min
绂诲績
15min
锛屼笂娓呮恫涓虹矖閰舵恫銆傜劧鍚庡湪璇曠涓?/p>
鍔?/p>
pH 7.0
鐨勭７閰哥紦鍐叉恫
2 ml
锛?/p>
鎰堝垱鏈ㄩ厷
锛?/p>
0.2%
锛?/p>
0.5ml
锛?/p>
娴撳害涓?/p>
0.15%
鐨?/p>
H
2
O
2
0.5ml
锛?/p>
鍙?/p>
0.3ml
鐨勯叾鎻愬彇娑插姞鍏ュ埌璇曠涓紝
绌虹櫧浠ョ紦鍐叉恫浠ｆ浛銆?/p>
鍦?/p>
470nm
涓嬫祴瀹氬叾鍚稿厜搴︼紝
鍔犲叆閰舵恫鏃跺紑濮嬭鏃讹紝姣忛殧
30s
璇绘暟涓�娆★紝杩炵画璁板綍
5
鍒嗛挓銆備互姣忓垎閽熸瘡鍏嬮矞閲嶅
鍔?/p>
0.1
鐨勯叾閲忎綔涓轰竴涓叾娲绘�у崟浣嶃�?/p>
鈻?/p>
470 脳
V
T
POD
娲绘�?/p>
=
0.01脳
t脳
Vs脳
W
寮忎腑锛?/p>
鈻?/p>
470----
鍙嶅簲鏃堕棿鍐呭惛鍏夊害鍊肩殑鍙樺寲锛?/p>
V
T
----
鎻愬彇閰舵恫鐨勬�讳綋绉紙
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t----
鍙嶅簲鐨勬椂闂达紙
min
锛?/p>
Vs ----
娴嬪畾鏃跺彇鐢ㄩ叾娑蹭綋绉紙
ml
锛?/p>
W----
鏍峰搧椴滈噸锛?/p>
g
锛?/p>
浜屻�佸閰氭哀鍖栭叾锛?/p>
PPO
锛夋椿鎬х殑娴嬪畾
澶氶厷姘у寲閰舵椿鎬ф祴瀹氬弬鐓ф湵骞垮粔绛?/p>
(1990)
鐨勬柟娉曪紝鐣ュ姞鏀瑰姩銆?/p>
閰舵恫鍒跺锛氱О鍙栨牱鍝?/p>
0.5g
锛屽姞鍏?/p>
5mL
0.1mol
锛?/p>
L PH=6.0
鐨勭７閰哥紦鍐叉憾娑?/p>
锛?/p>
鍐版荡鐮旂（鎴愭祮锛?/p>
4
鈩冩潯浠朵笅
12000r/min
绂诲績
15min
鍚庝笂娓呮恫鍗充负绮楅叾娑层�?/p>
PPO
娲绘�ф祴瀹氾細
鍙嶅簲璇曠涓垎鍒姞鍏?/p>
0.1 mL
閰舵恫
+3.9 mL
纾烽吀缂撳啿娑?/p>
+ 1 mL
1m mol
锛?/p>
L
鐨勯偦鑻簩閰氥�傛贩鍖�鍚庝簬
30
鈩冧繚娓?/p>
10 min
锛岃繀閫熷姞鍏?/p>
2 mL
璐ㄩ噺鍒嗘暟
涓?/p>
20
锛呯殑涓夋隘涔欓吀缁堟鍙嶅簲锛岀珛鍗充簬
525nm
涓嬫祴瀹氬叾鍚稿厜搴﹀�硷紝璁＄畻閰舵椿銆?/p>
PPO
娲绘�?/p>
=
O
D
脳
V
T
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t脳
0.5g脳
V
s
=
O
D
脳
V
T
0.005
OD
鈥斺�?/p>
鍙嶅簲鏃堕棿鍐呭惛鍏夊�肩殑鍙樺寲锛?/p>
V
T
----
鎻愬彇閰舵恫鐨勬�讳綋绉紙
ml
锛?/p>
Vs ----
娴嬪畾鏃跺彇鐢ㄩ叾娑蹭綋绉紙
ml
锛?/p>
T
鈥斺�斺�?/p>
鍙嶅簲鏃堕棿
(min)
锛?/p>